Other

Part:BBa_K2592006

Designed by: Janice Law   Group: iGEM18_Hong_Kong_HKU   (2018-09-23)
Revision as of 10:39, 28 September 2018 by Janice Law (Talk | contribs)


Strand 2 for in-vivo synthesis of Nano Drug Carrier-AS1411 (NDC-as)

This part encodes a component strand of our originally designed Nano Drug Carrier-AS1411 (NDC-as). This single strand encoded carries DNA aptamer AS1411. The strand-encoding region is followed by an HIV-terminator binding site (HTBS). This component strand can be expressed in the presence of HIV reverse transcriptase and murine leukaemia virus reverse transciptase inside E. coli DH5alpha (Elbaz, 2016).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None