Regulatory
Part:BBa_K2569011:Design
Designed by: wenxue Zhao Group: iGEM18_SKLMT-China (2018-09-27)
PpltR, constitutive promoter in Pseudomonas. fluorescence pf-5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Promoter PpltR is a constitutive promoter that can be used in regulating the gene expression in Pseudomonadaceae.
Source
It is taken from the upstream region of the gene pltR ,whose length is 70bp.It is one of the constitutive promoters of pseudomonas fluorescence promoter library,its strength is characterized by a reporter gene ,firefly luciferase.It can be used to regulate the gene expression in Pseudomonadaceae through 3A Assembly.