Composite

Part:BBa_K2740011

Designed by: Zhaoqing Hu   Group: iGEM18_Nanjing-China   (2018-09-26)
Revision as of 13:18, 26 September 2018 by Registry (Talk | contribs)

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Nitrogen fixation (nif) gene cluster of Paenibacillus polymyxa CR1

A gene cluster enables synthesis of catalytically active nitrogenase in wild type P. polymyxa CR1 and the accordingly genetically engineered E. coli. This cluster is organized as an operon comprising nine structure genes nifB, nifH, nifD, nifK, nifE, nifN, nifX, hesA and nifV. Besides, it contains the native promoter that located upstream of nifB and the native terminator that located downstream nifV respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 875
    Illegal PstI site found at 2887
    Illegal PstI site found at 6767
    Illegal PstI site found at 6925
    Illegal PstI site found at 7684
    Illegal PstI site found at 7878
    Illegal PstI site found at 10071
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 875
    Illegal PstI site found at 2887
    Illegal PstI site found at 6767
    Illegal PstI site found at 6925
    Illegal PstI site found at 7684
    Illegal PstI site found at 7878
    Illegal PstI site found at 10071
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 875
    Illegal PstI site found at 2887
    Illegal PstI site found at 6767
    Illegal PstI site found at 6925
    Illegal PstI site found at 7684
    Illegal PstI site found at 7878
    Illegal PstI site found at 10071
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 875
    Illegal PstI site found at 2887
    Illegal PstI site found at 6767
    Illegal PstI site found at 6925
    Illegal PstI site found at 7684
    Illegal PstI site found at 7878
    Illegal PstI site found at 10071
    Illegal NgoMIV site found at 4796
    Illegal NgoMIV site found at 5482
    Illegal NgoMIV site found at 7371
    Illegal AgeI site found at 5855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5046
    Illegal BsaI.rc site found at 8842
    Illegal SapI.rc site found at 2330
    Illegal SapI.rc site found at 5429


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
Parameters
proteins
strainPaenibacillus polymyxa CR1