Composite

Part:BBa_K2810011:Design

Designed by: Ryan Coates   Group: iGEM18_Cardiff_Wales   (2018-09-26)
Revision as of 09:58, 26 September 2018 by Registry (Talk | contribs)

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mCherry under control of the bacterial promoter and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was synthesised as a gBlock and was not put together from separate level 0 parts. This means unlike our other parts in the collection, it does NOT have the Type IIS restriction enzyme sites and sticky ends that make it GoldenGate compatible.


Source

Sequenced as a gBlock. The promoter is from X, the coding sequence originally RFP from Discosoma, and the promoter from X.

References