Coding

Part:BBa_K2599007

Designed by: YEN-LING CHEN   Group: iGEM18_NCTU_Formosa   (2018-09-16)
Revision as of 12:07, 25 September 2018 by Yen-ling (Talk | contribs)


Antimicrobial peptide - Durancin TW-49M

This biobrick is the basic part of the Durancin TW-49M, more information please look for the composite part (BBa_K2599015).



Figure 1. Basic part of Durancin TW-49M


Introduction

Durancin TW-49M produced by Enterococcus durans QU 49, is a nonpediocin-like class II bacteriocin. Enterococcus are highly competitive due to their resistance to wide range of pH and temperature. Therefore, the bacteriocins they produce shows their wide range effectiveness on pathogenic bacteria.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Durancin TW-49M sequence length is around 213 b.p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

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Categories
Parameters
None