Coding

Part:BBa_K2599004

Designed by: YEN-LING CHEN   Group: iGEM18_NCTU_Formosa   (2018-09-16)
Revision as of 11:59, 25 September 2018 by Yen-ling (Talk | contribs)


Antimicrobial peptide - Enterocin 96

This biobrick is the basic part of the enterocin 96, more information please look for the composite part (BBa_K2599012).



Figure 1. Basic part of Enterocin 96


Introduction

Enterocin 96, is a classII bacteriocin produced by Enterococcus faecalis. It has strong activity against most gram-positive strains but almost no activity against gram-negative strains, such as Escherichia coli. The antimicrobial spectrum is relatively wide compared with other bacteriocins from lactic acid bacteria.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The enterocin 96 sequence length is around 219 b.p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[edit]
Categories
Parameters
None