Coding

Part:BBa_K2599006

Designed by: YEN-LING CHEN   Group: iGEM18_NCTU_Formosa   (2018-09-16)
Revision as of 11:58, 25 September 2018 by Yen-ling (Talk | contribs)


Antimicrobial peptide - Leucocyclicin Q

This biobrick is the basic part of the Leucocyclicin Q, more information please look for the composite part (BBa_K2599014).



Figure 1. Basic part of Leucocyclicin Q


Introduction

Leucocyclicin Q, produced by Leuconostoc mesenteroides TK41401, possesses antimicrobial activity against broad range of bacteria srains, and particularly shows strong activity against Bacillus coagulans. Leucocyclicin Q was proved to be a cyclic bacteriocin in which N and C termini are bound to each other, and its structure is very similar to Lactocyclicin Q. Furthermore, it shows high stability against different pH value or heat stress.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Leucocyclicin Q sequence length is around 186 b.p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

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Categories
Parameters
None