Regulatory

Part:BBa_K2560007:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-08-20)
Revision as of 09:15, 25 September 2018 by Henselt (Talk | contribs) (→‎Design Notes)


Phytobrick version of BBa_J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was obtained from part BBa_J23100. The parts sequence was not changed apart from adding the promotor overhangs that are required for subsequent cloning.

<img style="width:50%" src="https://parts.igem.org/File:Promoter_characterization_Marburg_Toolbox.pdf#file">

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector (BBa_K2560002) using Golden Gate assembly.

Forward Oligo: CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACT

Reverse Oligo: CTCAAGTAGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCC

References