Generator

Part:BBa_K2609015

Designed by: Bhaskar Kumawat   Group: iGEM18_IISc-Bangalore   (2018-09-24)
Revision as of 09:56, 24 September 2018 by Registry (Talk | contribs)

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gp37 under T7 expression system - Mod IV

This part generates gp37, the tip of the T4 bacteriophage long receptor binding tail, when expressed in a T7 expression strain like Bl21 (DE3).

Contains amino acid modifications that predict an increased affinity for phosphoethanolamine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1239
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 507
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None