Generator

Part:BBa_K2609007

Designed by: Bhaskar Kumawat   Group: iGEM18_IISc-Bangalore   (2018-09-24)
Revision as of 09:24, 24 September 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


mcp-1 under T7 expression system

This part generates mcp-1 (a mouse monocyte chemokine) when expressed inside a T7 expression strain like Bl21 (DE3). The protein is fused to a 6xHis tag and a TEV protease cleavage site on the N-term.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 464
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None