Protein_Domain
Part:BBa_K2812000:Design
Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls Group: iGEM18_TU-Eindhoven (2018-09-23)
Carbohydrate-binding domain from MpIBP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
DNA sequence codon optimized for E. coli.
Source
Genomic sequence from M. primoryensis. Protein sequence obtained from Guo et al. 2017 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550230/)
References
Guo, S., Stevens, C., Vance, T., Olijve, L., Graham, L., Campbell, R., . . . Davies, P. (2017). Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice. Science Advances, 1-10.