Part:BBa_K2568006

Virus receptor Nectin 4 with Kozak sequence and FLAG-tag

The way in which viruses enter into cells is mainly mediated by the host receptor. If a cell can simultaneously express Nectin 4, TfR and other receptors, this cell can be infected by most viruses and is expected to solve the cultivation problem of most viruses. As a cell adhesion molecule, Nectin 4 plays a key role in the process of viruses invading cells. The chassis cell MDBK, which is a commonly used cell line in the production of bovine vaccines, has most of the essential receptors and lacks only Nectin 4 and TfR receptors. Nectin 4 is one of the receptors of canine distemper virus (CDV), and its lgV structure forms the strongest binding ability of all receptors with the H protein in CDV. When a plasmid containing a Nectin 4 fragment is transfected into MDBK cells and successfully expressed, the CDV can invade MDBK cells and then multiply.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 220
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1041
  Illegal BsaI.rc site found at 1369
  Illegal SapI.rc site found at 1168