Regulatory

Part:BBa_K2688010

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-21)
Revision as of 09:47, 23 September 2018 by Briand (Talk | contribs)

LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon, named LEE5.

This region has been shown to be recognized by two nucleoid-associated proteins, H-NS and Ler (‘LEE encoded regulator’). The regulation by these proteins can generate some heterogeneity of transgene expression within E. coli populations as recently shown by Leh and collaborators (Leh et al, mBIO, 2017).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 57
    Illegal NgoMIV site found at 745
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 844
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Categories
Parameters
None