Tag
LAA

Part:BBa_M0050:Design

Designed by: Felix Moser   Group: Endy Lab   (2007-12-05)
Revision as of 17:52, 5 December 2007 by Fmoser76 (Talk | contribs) (→‎Design Notes)


AANDENYALAA. (Very fast) SsrA degradation tag.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C-terminal tag. Degradation rate is very fast. Deviations from this sequence in key amino acids will lower degradation rates (see Parts BBa_M0051, BBa_M0052, BBa_M0053). Sequence derived from reverse translation of AANDENYALAA sequence using codon usage optimized for E. coli. Three C-terminal aa's (LAA in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX. See sources on the main part page for further information about the mechanism of the system.

NOTE: this sequence has only the amino acid sequence for the tag and bears NO STOP CODONS, so be sure to inlcude them if you use this sequence.

Source

C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli.

References