Coding
KaiA

Part:BBa_K2637001

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-09-20)
Revision as of 10:07, 20 September 2018 by GangGang (Talk | contribs)


KaiA Protein of Circadian Rhythm


KaiA is one of the essential proteins in the circadian rhythm of cyanobacterias. It can regulate the process of KaiC’s phosphorylation and dephosphorylation together with KaiB and other proteins in our system, and then drive the cyanobacterias into subjective dawn or dusk. We have optimized it in yeast.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 832
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Biology and Usage

Overview of Cyanobacterias' circadian rhythm

Usage of Life Tik Tok(Cyanobacterias' circadian rhythm in yeast)

About Life Tik Tok

Intereaction between the proteins

Reconstruction of Life Tik Tok in yeast

Origin of KaiA

Characterization of Life Tik Tok

Reporters' measurement in yeast

Characterization of Life Tik Tok

References

[edit]
Categories
//cds
//chassis/eukaryote/yeast
//direction/forward
Parameters
chassisS. cerevisiae
directionForward
functionKaiC's phosphorylation regulator