Coding
KaiA
Part:BBa_K2637001
Designed by: Haoqian Zhao Group: iGEM18_Tianjin (2018-09-20)
KaiA Protein of Circadian Rhythm
KaiA is one of the essential proteins in the circadian rhythm of cyanobacterias. It can regulate the process of KaiC’s phosphorylation and dephosphorylation together with KaiB and other proteins in our system, and then drive the cyanobacterias into subjective dawn or dusk. We have optimized it in yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 832
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Biology and Usage
Overview of Cyanobacterias' circadian rhythm
Usage of Life Tik Tok(Cyanobacterias' circadian rhythm in yeast)
About Life Tik Tok
Intereaction between the proteins
Reconstruction of Life Tik Tok in yeast
Origin of KaiA
Characterization of Life Tik Tok
Reporters' measurement in yeast
Characterization of Life Tik Tok
References
[edit]
Categories
Parameters
//cds
//chassis/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//direction/forward
chassis | S. cerevisiae |
direction | Forward |
function | KaiC's phosphorylation regulator |