Coding

Part:BBa_K2729000

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-18)
Revision as of 02:57, 19 September 2018 by Hajime Fujita (Talk | contribs)


DENV1 C (Capsid Protein | Dengue Virus Serotype I)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

- NCBI Gene Database


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例

Figure 1: Result of the qualitative experiment - Cells were grown at room temperature on LB agar plates with and without iP. β-galactosidase activity was monitored by X-gal. Photographs were taken after 25h incubation.


As shown in Fig. 1, blue color was developed only when cells carried the AHK4 expressing plasmid and when the medium contained 100 µM iP. Therefore, we concluded that AHK4 could receive iP and downstream AHK4→RcsD→RscB→cps::lacZ pathway was activated as expected.



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