Coding

Part:BBa_K2599000

Designed by: YEN-LING CHEN   Group: iGEM18_NCTU_Formosa   (2018-09-12)
Revision as of 09:06, 18 September 2018 by Yen-ling (Talk | contribs)


Antimicrobial peptide - Subtilosin

This biobrick is the basic part of the Subtilosin, more information please look for the composite part (BBa_K2599008).


Figure 1 biobrick picture

Introduction

Bacillus subtilis produces a bacteriocin called Subtilosin that possesses antibacterial activity against certain gram-positive bacteria. The bacteriocins are a group of anitmicrobial peptides that are often distinguished from traditioinal antibiotics by their narrow range of avtivity against closely related bacteria.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Subtilosin sequence length is around 147 b.p.


Figure 2 PCR




Reference



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None