Composite

Part:BBa_K2587018

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)
Revision as of 19:47, 17 September 2018 by Registry (Talk | contribs)

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Prpa_RBS_E_T

This construct contains lysis gene E is a lysis gene from the bacteriophage PhiX174E. This gene is used in our co-culture to induce self lysis of E.coli under the control of the pRpa promoter. This promoter is part of the Quorum sensing system of Rhodopseudomonas palustris . Together with the other components of the system, RpaR and RpaI , this promoter will induce expression of the lysis gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93


[edit]
Categories
Parameters
None