Part:BBa_K2599008
T7 Promoter+RBS+Subtilosin+intein+CBD
NCTU_Formosa 2018 designed a composite part encoding the Subtilosin A sequence (BBa_K2599000), and then combined with a T7 promoter (BBa_I712074), a ribosome binding site (BBa_B0034), intein and chintin binding domain (CBD).
Figure 1 biobrick picture
Introduction
Subtilosin A is produced by Bacillus subtilis and belongs to the bacteriocin class V family. Bacteriocins are proteinaceous antimicrobial substances that are often distinguished from traditional antibiotics by their narrow range of avtivity against closely related bacteria. It had bacteriocidal activity against some gram-positive bacteria such as Listeria, some species of Bacillus and E.faecium.
Structure Analysis
The amino acid was determined to be as follows:X-Gly-Leu-Gly-Leu-Trp-Gly-Asn-Lys-Gly-Cys-Ala-Thr-Cys-Ser-(sequence; see text) Ile-Gly-Ala-Ala-Cys-Leu-Val-Asp-Gly-Pro-Ile-Pro-Asp-Glx-Ile-Ala-Gly-Ala. The analyses of cross-linking structures revealed that there were linkages between the amino- and carboxyl-termini and between the Cys-19 and the Glx-28 residues through an unknown residue with a residue weight of 163. Consequently, subtilosin A was deduced to be a cyclic peptide antibiotic with a novel cross-linking structure.
Figure 2 structure picture
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1055
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 778
Illegal AgeI site found at 868 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 698
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