Composite

Part:BBa_K2533048:Design

Designed by: kangyuan yu   Group: iGEM18_HUST-China   (2018-09-15)
Revision as of 06:36, 15 September 2018 by Registry (Talk | contribs)

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RBS-mleS-RBS-lldP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 212
    Illegal BglII site found at 1942
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 529
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 904
    Illegal NgoMIV site found at 1075
    Illegal NgoMIV site found at 1698
    Illegal NgoMIV site found at 2064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 936
    Illegal BsaI site found at 2435
    Illegal BsaI.rc site found at 388
    Illegal BsaI.rc site found at 676
    Illegal BsaI.rc site found at 1243
    Illegal BsaI.rc site found at 1588
    Illegal BsaI.rc site found at 2331


Design Notes

no


Source

Lactococcus lactis subsp. lactis Il1403 & Escherichia coli str. K-12 substr. MG1655

References