Coding
Part:BBa_K2586003
Designed by: Robert Warneke, Janek Meißner Group: iGEM18_Goettingen (2018-09-10)
Revision as of 11:11, 13 September 2018 by Dschannek96 (Talk | contribs)
aroE: 3-phosphoshikimate 1-carboxyvinyltransferase
This part encodes for the 3-phosphoshikimate 1-carboxyvinyltransferase.
The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. It is essential in Bacillus subtilis , as three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr.
aroE encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application.
An amplification of aroE allows for glyphosate resistance, this is used in multiple GM plants to increase the yield and improve farming applications.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 90
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 415
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/bsubtilis
target |