Translational_Unit
Csy4-Q104A

Part:BBa_K2615004:Design

Designed by: Yunqian Zhang   Group: iGEM18_OUC-China   (2018-09-01)
Revision as of 12:31, 11 September 2018 by Anyi (Talk | contribs) (→‎Design Notes)


Csy4-Q104A, the No.2 member of Csy4 family.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 93


Design Notes

We designed this part by point mutation. We changed the CAG(encoding Gln) to GCG(encoding Ala) on the 104th site based on wild type Csy4.

Source

  The cleavage of Cys4 releases a cis-repressive RNA module (crRNA,paired with RBS) from the masked ribosome binding site (RBS), which subsequently allows the downstream translation initiation. A Ribosome Binding Site (RBS) is an RNA sequence to which ribosomes can bind and initiate translation. Combined RBS and Cys4, it can be more convenient for other iGEMers to use this composite part without putting a RBS aequence on the upstream of  Cys4 coding sequence.

References