Composite

Part:BBa_K2573000

Designed by: Amanda Kuang   Group: iGEM18_Waterloo   (2018-08-03)
Revision as of 18:57, 10 September 2018 by Froggyinthewater (Talk | contribs)


MetE Coding Device


MetE codes for the methyl-transferase for methionine synthesis. Since methionine is required for E.coli growth, by introducing the MetE gene into plasmid desired for insert it can be used to differentiate between E.coli cells that have successfully taken up the insert and those that have not in a medium lacking methionine.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1864
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//plasmid
Parameters
None