Part:BBa_K1062004:Experience

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UNIQfd6c71ae05075411-partinfo-00000000-QINU

••• USTC_China iGEM13

In 2018 OUC-China's project, we used csy4 as a main role in our toolkit. We design a toolkit focused on translational regulation, which is composed of a RNA endoribonuclease (Csy4) and a RNA module (hairpin). Csy4 (Csy6f), a member of CRISPR family, recognizes a specific 16-nt nucleotide repetitive sequence(hairpin). The RNA module was constructed by inserting the 16nt Csy4 recognition site between a RBS and cis-repressive RNA element, which can be specifically cleaved upon Csy4 expression, so that the RBS is masked. Cleaved at the specific recognition site, it can release the masked RBS, thus endowing the programming of gene expression in the translation level with higher feasibility. We found that csy4 is a perfect part for our toolkit. Because once the RNA/Csy4 complex formed, the structure become really stable and hard to separate which make our toolkit stable when it function.