Intermediate

Part:BBa_S03724

Designed by: Josh Michener   Group: iGEM04_MIT   (2007-06-26)
Revision as of 06:23, 27 October 2007 by Cal (Talk | contribs)

B0015:S03708 A weak (as compared to J231) constitutive promoter J23116 produces a steady stream of the tet repressor protein. When cloned into other constructs containing pTet, this tetR will constantly repress transcription of the pTet-regulated gene. This intermediate also contains a B0015 terminator upstream of the tetR producing region, so that pTet-spacer-cro/N/Q/YFP constructs can be easily cloned upstream of the terminator, resulting in the final testing construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None