Composite

Part:BBa_K2539200

Designed by: Catherine Chang   Group: iGEM18_TAS_Taipei   (2018-09-03)
Revision as of 09:35, 3 September 2018 by Registry (Talk | contribs)

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ALDH2*2 Expression Construct

A regulated ALDH2*2, a mutant form of ALDH2*1, expressing construct that employs the ethanol and alcR induced promoter (BBa_K2092002) and a strong RBS (BBa_B0034) to regulate expression of ALDH2*2, a mitochondrial enzyme responsible for the oxidation of acetaldehyde into acetate. The sequence is then terminated with a double terminator (BBa_B0015) to ensure the end of transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 366
    Illegal NgoMIV site found at 509
    Illegal NgoMIV site found at 1022
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 428
    Illegal SapI.rc site found at 925


[edit]
Categories
Parameters
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