Composite

Part:BBa_K2539101

Designed by: Catherine Chang   Group: iGEM18_TAS_Taipei   (2018-09-03)
Revision as of 09:07, 3 September 2018 by Registry (Talk | contribs)

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ALDH2*1 + 6XHIS Expressing Construct

A composite part that includes the strong promoter/strong RBS (K880005) combination to upregulate expression of ALDH2*1, a mitochondrial enzyme that oxidizes acetaldehyde into acetate with a HIS tag that will purify the ALDH2*1 enzyme.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 393
    Illegal NgoMIV site found at 536
    Illegal NgoMIV site found at 1049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 455
    Illegal SapI.rc site found at 952


[edit]
Categories
Parameters
None