Composite

Part:BBa_K2539300

Designed by: Justin Wu   Group: iGEM18_TAS_Taipei   (2018-09-03)
Revision as of 09:00, 3 September 2018 by Registry (Talk | contribs)

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AlcR Expression Construct

An alcR-based construct that employs the strong promoter/strong RBS (K880005) combination to upregulate expression of alcR, a regulatory protein that, along with substances like ethanol, activates PalcA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2611
    Illegal BamHI site found at 1615
    Illegal BamHI site found at 2353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1319
    Illegal AgeI site found at 1447
    Illegal AgeI site found at 1978
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None