Coding
Part:BBa_K2638001
Designed by: Erika Schneider Group: iGEM18_Bielefeld-CeBiTec (2018-09-02)
CopC coding sequence
CopC is a gene that encodes for a soluble protein within the periplasmic space(β-barrel structure) with a highly specific Cu(I) and another highly specific Cu(II) binding site. It is part of the CopABCD gene cluster. CopC in Pseudomonas syringae pathovar tomato promotes Cu(II) uptake into the periplasmic space. It was used a homologue from Pseudomonas brassicacearum 3Re2-7 13. Together with CopD (BBa_K2638002) it promotes copper uptake from outside the cell into the cytoplasm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 304
Illegal AgeI site found at 358 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 246
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Categories
Parameters
None |