RNA

Part:BBa_K2624004

Designed by: Shuyao Zhou   Group: iGEM18_CPU_CHINA   (2018-08-26)
Revision as of 08:12, 26 August 2018 by Registry (Talk | contribs)

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U6-inhSi-pri-miRNA

The corresponding short transcript of RNA would base-pair with the basal segments of the designed pri-miRNA analogue so that the pri-miRNAs cannot be recognized and processed to the corresponding pre-miRNAs. But it also contains a RNA promoter for the RNA-dependent RNA polymerase NS5B. Upon substitutional hybridation catalyzed by the polymerase, the inhibitory strand unbinds with the pri-miRNA so that the RNA interference takes effect.
Please refer to the wiki of CPU_CHINA 2018 because this part is strictly related to the other parts used in the project. You may need it if you follow their conditional RNA interference strategy in your study.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 312
    Illegal NgoMIV site found at 341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 305


[edit]
Categories
//rna/ribozyme
Parameters
None