Composite

Part:BBa_K2624005:Design

Designed by: Shuyao Zhou, Huandi Xu, Junqing Zhang, Yali Wu   Group: iGEM18_CPU_CHINA   (2018-08-17)
Revision as of 09:02, 17 August 2018 by Registry (Talk | contribs)

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hulc-pri-miRNA(MAP4K4)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 130


Design Notes

We checked the exact transcription start site of the hulc gene and replaced the sequence after it with the sequence that we designed so that, instead of the lncRNA, it would express the pri-miRNA analogue. Then of course we added a SV40 poly(A) sequence that signals the end of a transcriptional unit. In the end we added 5 different restriction sites for 5 different endonucleases, three in the front and two at the end, for recombination convenience.


Source

Hulc promoter sequence comes from Swissregulon Database. The pri-miRNA analogue part is designed by us.

References