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Part:BBa_K2560002:Design

Designed by: Daniel Stukenberg   Group: iGEM18_Marburg   (2018-08-13)
Revision as of 13:03, 13 August 2018 by DanielStukenberg (Talk | contribs) (→‎References)


Phytobrick Entry Vector with GFP dropout


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1035
    Illegal SapI.rc site found at 193


Design Notes

More information to come


Source

The Phytobrick Acceptor Vector BBa_P10500 was used as a Template. LacZ cassette was replaced by sfGFP cassette which was PCR amplified from pYTK001 (Lee et al. 2015) and cloned into the vector with Gibson Assembly.

References

Lee M. E., DeLoache W. C., Cervantes B., Dieber J. E. (2015) A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly, ACS Synth. Biol., 4(9):975-986