Other
Part:BBa_K2560002:Design
Designed by: Daniel Stukenberg Group: iGEM18_Marburg (2018-08-13)
Phytobrick Entry Vector with GFP dropout
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1035
Illegal SapI.rc site found at 193
Design Notes
More information to come
Source
The Phytobrick Acceptor Vector BBa_P10500 was used as a Template. LacZ cassette was replaced by sfGFP cassette which was PCR amplified from pYTK001 (Lee et al. 2015) and cloned into the vector with Gibson Assembly.