Other
Part:BBa_K2560001:Design
Designed by: Daniel Stukenberg Group: iGEM18_Marburg (2018-08-13)
Phytobrick Entry Vector with RFP dropout
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 51
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 21
Illegal NheI site found at 44
Illegal SpeI site found at 51 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 51
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 51
Illegal AgeI site found at 630
Illegal AgeI site found at 742 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 927
Design Notes
More information to come
Source
The Phytobrick Acceptor Vector BBa_P10500 was used as a Template. LacZ was replaced by BBa_E1010 (RFP CDS)using Gibson Assembly