Coding

Part:BBa_K2688000:Design

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-08-01)
Revision as of 15:57, 1 August 2018 by Briand (Talk | contribs) (→‎Source)

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cpg2_cytosolic


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 57
    Illegal NgoMIV site found at 745
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 844


Design Notes

This CDS was edited by removal of the signal peptide, allowing a cytosolic location instead of the uusual periplasmic location.

This CDS was codon optimized for E. Coli. (original sequence had higher GC and differing codon bias).

The sequence was designed so that the protein product is identical to the drug Voraxase(r) (INN: glucarpidase) used in methotrexate poisonning.


Source

Cpg2 is an enzyme originally isolated from Pseudomonas strain RS-16.

References