Device

Part:BBa_J119409:Design

Designed by: Sofia Aguilera, Anthony Eckdahl, Nicklaus Hanlan, Ky Roland   Group: Eckdahl Lab   (2018-06-07)
Revision as of 15:32, 13 July 2018 by Eckdahl (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


SmaI Restriction Endonuclease Expression Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 299
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 641


Design Notes

ApaI sites were introduced at the beginning and the end of the construct for connection to the scaffold. BioBrick enzymes and BsaI sites were avoided. Deleted G in position 300 of the construct.

Source

Synthetic oligonucleotides traditionally cloned using pSB1A7.

References