Part:BBa_K2680251

3G mScarlet-I Coding Sequence for red fluorescent protein mScarlet-I in Team William_and_Mary iGEM 2018's 3G format. This part is also compatible with CIDAR MoClo assembly. mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 . mScarlet-I is distinguished from the original mScarlet protein by a single amino acid substitution (T74I). mScarlet-I has a peak excitation at 569nm and peak emission at 594nm, and possesses both a high quantum yield (0.54) and fluorescence lifetime (3.1ns), and a rapid maturation time of ~36 minutes [1].

References

[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M. Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 574
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 49
  Illegal BsaI.rc site found at 778