Coding

Part:BBa_M50439:Experience

Designed by: Adam C Elliott   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 11:58, 12 June 2018 by Aelliott (Talk | contribs) (→‎Stanford Location)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M50439

This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.

User Reviews

UNIQf824afa001a55c07-partinfo-00000000-QINU UNIQf824afa001a55c07-partinfo-00000001-QINU

This plasmid was used in Bba_M50440 and the user experience is better outlined there.

Stanford Location

Plasmid name: pHlash
DNA2.0 Gene #: pD1204-NSH
Organism: S. cerevisiae
Device type: Sensor
Glycerol stock barcode: 133021752
Box label: BioE44 S18