Project
IPTG Induc

Part:BBa_I741002:Experience

Designed by: Lane Weaver   Group: iGEM07_Penn_State   (2007-06-07)
Revision as of 00:16, 27 October 2007 by LEWeiss (Talk | contribs) (→‎User Reviews)

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Please enter how you used this part and how it worked out.

Applications of BBa_I741002

The IPTG receiver complements a motB knockout strain RP3087 (in the presence of IPTG).
It is described in: Badalamenti, J. P., L. E. Weiss, C. J. Buckno, T. L. Richard, P. S. Weiss, and P. C. Cirino. 2007. Synthetic sports: a bacterial relay race. IET Synthetic Biology 1:61-63. [http://scitation.aip.org/getpdf/servlet/GetPDFServlet?filetype=pdf&id=ISBEBU0000010001-2000061000001&idtype=cvips&prog=normal PDF]
This construct was chosen out of 15 combinations of RBS and promoters available at Penn State. Only the two strongest RBS and Promoter combinations were able to repress motB to effectively eliminate motility.


I have not experienced trouble with this part.

User Reviews

UNIQe0cbbbaa0a843eec-partinfo-00000000-QINU UNIQe0cbbbaa0a843eec-partinfo-00000001-QINU