Coding

Part:BBa_M50435:Design

Designed by: Eva Frankel, Kelsie Wysong, Lillian Zhu   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 06:08, 12 June 2018 by Lilzhu8 (Talk | contribs) (→‎Source)

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entC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The entC nucleotide sequence was obtained by codon-optimizing (for E. coli) the amino acid sequence obtained from UniProt, using IDT's reverse translation tool. A 6xHis tag was appended to the C terminus for antibody detection and assay purposes (Western Blot, ELISA, etc.)


Source

Organism: E. coli
UniProt amino acid sequence: https://www.uniprot.org/uniprot/P0AEJ2
6xHis: BBa_K133133

References

https://www.uniprot.org/uniprot/P0AEJ2
https://parts.igem.org/Part:BBa_K133133
Ma, L. & Payne, S. “AhpC is Required for Optimal Production of Enterobactin by Escherichia coli”. Journal of Bacteriology, 2012. http://jb.asm.org/content/194/24/6748.long