Project

Part:BBa_M50447:Design

Designed by: Demetri Samuel Maxim, Tom Gold   Group: Stanford BIOE44 - S11   (2018-06-12)
Revision as of 05:48, 12 June 2018 by Registry (Talk | contribs)

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Glucose sensitive promoter attached to single chain insulin with his-tag and gfp reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3274
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3676
    Illegal BsaI.rc site found at 2086
    Illegal BsaI.rc site found at 3203
    Illegal SapI site found at 588
    Illegal SapI site found at 2187


Design Notes

It was long and took a while to be synthesized and sequenced.


Source

Designed by us, codon optimized for yeast.

References