Project

Part:BBa_M50447

Designed by: Demetri Samuel Maxim, Tom Gold   Group: Stanford BIOE44 - S11   (2018-06-12)
Revision as of 05:48, 12 June 2018 by Registry (Talk | contribs)

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Glucose sensitive promoter attached to single chain insulin with his-tag and gfp reporter

This was a project trying to use a glucose sensitive promoter to express insulin and EGFP given high concentrations of glucose. It did not work as intended, i.e. we saw no increase in insulin/egfp expression given increase in concentrations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3274
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3676
    Illegal BsaI.rc site found at 2086
    Illegal BsaI.rc site found at 3203
    Illegal SapI site found at 588
    Illegal SapI site found at 2187


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Categories
Parameters
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