Composite

Part:BBa_M50420:Design

Designed by: Sruthi Raguveer   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 04:26, 12 June 2018 by Raguveer (Talk | contribs)

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Mosquito Larvae Insecticide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 188
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized the Cry11Aa amino acid sequence for E. coli. All of our parts were sourced from existing iGEM parts which are listed below. We did not make any other modifications from the source sequences.


Source

The source is previously established parts and the protein from uniprot cry11Aa.

Promoter: BBa_K914003 https://parts.igem.org/wiki/index.php?title=Part:BBa_K914003

RBS: BBa_B0034 https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034

Leader Sequence: BBa_J32015 https://parts.igem.org/Part:BBa_J32015

Gene: BBa_K332011 codon optimized for E. coli https://parts.igem.org/Part:BBa_K332011

6x-His Tag: BBa_K1223006 https://parts.igem.org/Part:BBa_K1223006

Terminator: BBa_B0012 https://parts.igem.org/Part:BBa_B0012

References

http://pmep.cce.cornell.edu/profiles/insect-mite/mevinphos-propargite/naled/insect-prof-naled.html Höfte H and Whiteley H R, 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Reviews, 53(2), 242–255.

Delécluse, A., Rosso, M. L., & Ragni, A. (1995). Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein. Applied and environmental microbiology, 61(12), 4230-4235.