Part:BBa_M50436:Experience
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_M50436
This protein can be used to speed up enterobactin synthesis slightly in E. coli. It works in tandem with entC (BBa_M50435), facilitating the transformation of chorismate to isochorismate.
User Reviews
UNIQ3fa25ddb53d02dbe-partinfo-00000000-QINU UNIQ3fa25ddb53d02dbe-partinfo-00000001-QINU
We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ordered the plasmid from DNA2.0 and transformed it into E. coli, then plated the E. coli on LB + kanamycin. We saw colonies successfully grow up. We also ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized. See https://parts.igem.org/Part:BBa_M50437:Experience for a detailed assessment.