Composite

Part:BBa_M50437:Design

Designed by: Lillian Zhu, Kelsie Wysong, Eva Frankel   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 23:10, 11 June 2018 by Lilzhu8 (Talk | contribs) (→‎Source)

2,3-DHB Biosynthesis Construct, with entC and ahpC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 234
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our plasmid contained two distinct coding sequences, separately delineated with ribosome-binding sites.

Source

T5 promoter: BBa_M50075
Strong RBS: BBa_M50080
entC: BBa_M50436
ahpC: BBa_M50435
T7 terminator: BBa_M50080