Composite
pHlash

Part:BBa_M50440:Experience

Designed by: Adam C Elliott   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 17:45, 11 June 2018 by Aelliott (Talk | contribs)


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Applications of BBa_M50440

This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.

User Reviews

UNIQ4fdc86f2efc007f9-partinfo-00000000-QINU UNIQ4fdc86f2efc007f9-partinfo-00000001-QINU

Stanford Location

Plasmid name: pHlash
DNA2.0 Gene #: custom version of
Organism: S. cerevisiae
Device type: Sensor
Glycerol stock barcode: 133021752
Box label: BioE44 S18