Generator

Part:BBa_I718008:Experience

Designed by: David Bikard   Group: iGEM07_Paris   (2007-10-26)
Revision as of 00:03, 27 October 2007 by David.bikard (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I718008

This Cre recombinase generator can be used to induce recombination between two lox site at a tunable rate. An experience conducted by iGEM Paris 2007 gives the recombination rate without induction:

A strain bearing the cassette lox-kanR-lox inserted into its chromosome was transformed with BBa_I718008 in the pSB1A2 plasmid. Directly after transformation, liquid cultures were launched with either Amp or Amp+Kan. 100µl of the cultures were regularly plated at appropriated concentrations on LB+Amp, giving the following growth curves:

600px
Paris RecombinationRate.JPG

User Reviews

UNIQ68707794e25d4635-partinfo-00000000-QINU UNIQ68707794e25d4635-partinfo-00000001-QINU