Project
HSL Receiv

Part:BBa_I741001:Experience

Designed by: Jon Badalamenti, Lane Weaver, Tony Tascone, Lucien Weiss   Group: iGEM07_Penn_State   (2007-05-26)
Revision as of 23:58, 26 October 2007 by LEWeiss (Talk | contribs) (→‎Applications of BBa_I741001)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I741001

The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL. The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending). Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%). Confirmation of AHL production can be achieved by visualization of swarming (shown below).

Swarm Radius




User Reviews

UNIQ976f87f155af5796-partinfo-00000000-QINU UNIQ976f87f155af5796-partinfo-00000001-QINU I have been working with this part for several months, in which it has consistently worked. Leaky expression does appear to be a problem after 24+ hours, but it is quite stable until then.
note: the receiver construct is currently hosted on a psb2K3 plasmid.