Composite
pHlash

Part:BBa_M50440

Designed by: Adam C Elliott   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 00:18, 26 April 2018 by Registry (Talk | contribs)

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bioluminescent "pHlash" reporter for intracellular pH

This is a genetically encodable pH probe that will report cystolic pH in vivo of mammalian, yeast, or E. coli cells, utilizing Bioluminescence Resonance Energy Transfer (BRET). The fluorophore protein luminesces ratiometrically dependent on the pH in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 759
    Illegal AgeI site found at 1648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1500
    Illegal BsaI.rc site found at 1194
    Illegal SapI.rc site found at 1513


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Categories
Parameters
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