Coding
Part:BBa_M50439
Designed by: Adam C Elliott Group: Stanford BIOE44 - S11 (2018-04-25)
coding sequence bioluminescent "pHlash"reporter for intracellular pH
This is a genetically encodable pH probe that will report cystolic pH in vivo of mammalian, yeast, or E. coli cells, utilizing Bioluminescence Resonance Energy Transfer (BRET). The fluorophore protein luminesces ratiometrically dependent on the pH in the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 204
Illegal AgeI site found at 1093 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 945
Illegal BsaI.rc site found at 639
Illegal SapI.rc site found at 958
[edit]
Categories
Parameters
None |