Coding

Part:BBa_M50439

Designed by: Adam C Elliott   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 00:15, 26 April 2018 by Registry (Talk | contribs)

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coding sequence bioluminescent "pHlash"reporter for intracellular pH

This is a genetically encodable pH probe that will report cystolic pH in vivo of mammalian, yeast, or E. coli cells, utilizing Bioluminescence Resonance Energy Transfer (BRET). The fluorophore protein luminesces ratiometrically dependent on the pH in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 204
    Illegal AgeI site found at 1093
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 945
    Illegal BsaI.rc site found at 639
    Illegal SapI.rc site found at 958


[edit]
Categories
Parameters
None